绿矾对防治苹果黄叶病效应的初步研究

以适当浓度的綠矾溶液;或以綠矾——檸檬酸混合溶液浸泡病株树根,效果显著,病株复綠快速,惟不能持久。若以綠矾——有机混合肥料施于根区,防治效果良好,效力持久,但复綠較慢。综合利用这两种施用方法,卽:先用綠矾溶液浸泡树根;后用綠矾——有机混合肥料施于根区,則可达到防病快速持久的目的。     

Determination of plasma lysosomal enzyme activity and its relationship to severity of injury following blunt vehicular trauma in dogs

Objective: To determine plasma β‐d ‐glucuronidase (βG) activity in the first 4 hours following injury in dogs struck by a motor vehicle, and to evaluate whether the degree of enzyme activity is correlated with the severity of injury. Design: A prospective clinical study. Setting: Veterinary Medical Teaching Hospital. Animals: Thirteen client‐owned dogs that were presented to the Veterinary Hospital of the University of Pennsylvania between June and August 1999 for blunt vehicular trauma. Ten healthy student and staff‐owned dogs served as controls. Interventions: None. Measurements: Plasma was analyzed for βG enzyme activity at the time of presentation (n=13), and 1 and 4 hours (n=7) following presentation to the Emergency Service for blunt vehicular trauma. The results were compared with enzyme activity from healthy controls evaluated serially over 4 hours. Fluorometric analysis using 96‐well microtiter plates was used to perform the enzyme assays. The relationships between presentation (n=13) and 4 hours (n=7) of enzyme activity and 3 indices of metabolic and physical disturbance (serum pH, serum lactate and Animal Trauma Triage (ATT) score) at the time of presentation were also investigated. Main results: Of the 13 dogs, 7 fulfilled the inclusion criteria for comparison of enzyme activity of the trauma over time. A statistically significant difference in βG activity was found in the trauma group (mean 75.6±10.4 U) at 4 hours following presentation compared with controls (mean 48.0±6.4 U). This difference was suggested by 1 hour following presentation (trauma group, mean 70.4±10.9 U; control group, mean 49.8±5.5 U), although it did not reach statistical significance. Thirteen dogs fulfilled the inclusion criteria for comparison of only presentation enzyme activity with trauma severity score, serum lactate, and serum pH. No statistically significant relationship was found between the βd ‐glucuronidase activity and the presenting ATT score, serum lactate concentration, or serum pH at either presentation or 4 hours, although the power of these analyses was low. Conclusions: These results demonstrate that the activity of βG, a lysosomal enzyme, increases significantly in the systemic circulation in dogs 4 hours following blunt trauma. Additional research to include more severely injured dogs, a larger number of dogs, and to follow the course of injury for a longer period of time would be beneficial to further characterize βG activity following blunt trauma.     

Seroprevalence of and agroecological risk factors for Mycobacterium avium subspecies paratuberculosis and neospora caninum infection among adult beef cattle in cow-calf herds in Alberta, Canada

A province-wide cross-sectional seroprevalence and agroecological risk factor study of Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) infection among cattle in 100 cow-calf herds in Alberta was conducted. The seroprevalence of MAP in adult cattle was 1.5% across all herds. Using a widely accepted herd test cutpoint of 2 or more seropositive cows out of 30 animals tested, 7.9% of herds were estimated to be infected (95% confidence interval (CI): 2.3-23.4%). Seroprevalence of MAP differed by agroecological region; specifically, cattle and herds in areas with high soil pH (> 7.0), southern latitudes, and arid climates had a moderately reduced risk of infection (P < 0.10). Seroprevalence of NC infection was 9.7% among adult beef cattle province-wide--these levels also varied by agroecological region--with 91.0% of herds infected overall.     

Clean water: Factors that influence Its availability,quality and its use: Summary of the International Water Conference

The International Water Conference held in La Jolla, California during 28–30 November 1995, had an objective to provide an opportunity for international information exchange on new research applications and timely policy issues effecting the availability, use and quality of water for power generation. This Conference included the topical areas, the use of water and the management of chemical quality in process wastewaters; physical and biological transformation due to discharges in open surface waters, watersheds and wetlands; the role of risk and cost-benefit assessments; and discussion of the Clean Water Act containments. This Conference Summary contains an overview of the present state of the knowledge regarding the use of water by the electric utility industry and potential future issues as discussed in papers presented at the Conference and a summary of the most important findings and conclusions.     

Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow

Dendritic cells (DCs) are a heterogeneous population of cells of fundamental importance in initiating innate as well as specific immune responses. The identity and function of DCs in the cat are unknown, although they are likely pivotal in the response to infection. In this study, feline DCs were derived by 3-10-day culture of adherent blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) in the presence of IL 4 and GM-CSF. BMMC consistently yielded a greater number of DCs than PBMC, and there were fewer macrophages than DC from both compartments. DCs expressed a distinct constellation of surface molecules, which included CD1a, CD1b, and CD1c, CD11b, CD14, and 2-3-fold higher levels of MHC class I and II molecules than co-cultured macrophages or fresh blood monocytes. DCs displayed typical cytoplasmic processes, limited non-specific esterase activity, and acquired antigen by phagocytosis, pinocytosis, and binding to specific receptors. Cytokine-exposed cells induced proliferation of allogeneic lymphocytes. Thus, the cells derived by these culture conditions had markers and functions analogous to immature myeloid DCs. Availability of feline DCs will enable investigation of their role in infectious disease and their potential therapeutic application.     

美国松狮领军人——Imagine犬舍主理人Kathy&George

对松狮这个品种情有独钟的人一定知道美国最顶尖的松狮犬舍之——Imagine犬舍,美国国家认可的松狮俱乐部等级中首屈一指的犬舍。他们的松狮犬以帝王的外形、健全的身体和头脑而著称。Imagine犬舍坐落在美国南加州的圣贝纳迪诺山脚下,松树、桉树、棕榈树与盛开的丝兰和仙人掌围绕着他们美丽的家园,不远处就是积雪覆盖的山脉,绿、蓝、白的交会,组成了—幅美丽的画面。而对于犬舍主理人Kathy Beliew女士和她的丈夫George,更是广大松狮迷眼中偶像级的人物。